Development of Histological Techniques for Metabolic Syndrome Studies

Histology is an important branch in biology studies by studies the microscopic structures of human, animal and plant tissues. By histology, biologist can understand the biological changes that occur in cells by any disses lead to organ dysfunction such as metabolic syndrome. This study evaluates the biological changes that occur in tissue by metabolic syndrome. Haematoxylin and eosin staining was used to determine inflammatory cells and fat deposition in heart and liver. Further, a picrosirius red stain was used to distinguish the collagen deposition in heart. The tissues were taken from rats which fed by either a corn starch–rich (C) or highcarbohydrate, high-fat (H) diet for 16 weeks, and the C or H were supplemented with 0.8 g/kg quercetin diet for 8 weeks as treatment to metabolic syndrome, so the rats groups will be CQ and HQ. Histology is useful in many sectors such as health sector; for example it use hospital to diagnosis the diseases such as tumors and cancers. Also, it use in researches that related to health sector to help a researchers in that see the biological changes in tissue to confirm the results that obtain from the experiment. For instant, in this study by histology we see what the biological effects for quercetin in metabolic syndrome rats model. Therefore, by this way we can confirm that quercetin reduced the symptoms of metabolic syndrome such as gollagen deposition, fat deposition, and infiltrated inflammatory cells in liver and heart or quercetin has not any effect on symptoms of metabolic syndrome

Keywords: Histological techniques, Rats, high-carbohydrate diet, high-fat diet, Quercetin diet, Metabolic syndrome.